目前的金属偶联操作步骤复杂且耗时，并可能导致抗体回收率较低。

Expedeon的新产品Lightning-Link Metal Labeling Kits耗时短（可以在30秒内建立金属/抗体标记）,操作简单（只需将抗体溶液添加到激活的金属配体即可）。

操作步骤：

特点和优势：

手工操作只需30s

1h即可完成标记

一次只需10μ或100μg的标记物标记您的抗体

无分离步骤，100%抗体回收率

温和的化学反应，稳定的偶联物质

良好的批间重复性

应用广泛

规格：

1x 10 µg

1x 100 µg

10种供选择的金属元素：

139La, 141Pr, 144Nd, 151Eu, 153Eu, 154Sm, 159Tb, 165Ho, 169Tm, 175Lu

应用：

X射线荧光

质谱流式细胞术

多重离子束成像

解离放大镧系荧光免疫分析

应用举例：

Figure 1。 Goat Anti-Rabbit was labeled with Lightning-Link^® 159Tb at 100 µg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.

Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link^® metal labels at 10 µg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue™ (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.

Figure 3. X-Ray Fluorescence Lα series peaks used to quantify the metal content in Lightning-Link^® Metal Labeling kit.

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