目前的金属偶联操作步骤复杂且耗时,并可能导致抗体回收率较低。
Expedeon的新产品Lightning-Link Metal Labeling Kits耗时短(可以在30秒内建立金属/抗体标记),操作简单(只需将抗体溶液添加到激活的金属配体即可)。
操作步骤:
特点和优势:
手工操作只需30s
1h即可完成标记
一次只需10μ或100μg的标记物标记您的抗体
无分离步骤,100%抗体回收率
温和的化学反应,稳定的偶联物质
良好的批间重复性
应用广泛
规格:
1x 10 µg
1x 100 µg
10种供选择的金属元素:
139La, 141Pr, 144Nd, 151Eu, 153Eu, 154Sm, 159Tb, 165Ho, 169Tm, 175Lu
应用:
X射线荧光
质谱流式细胞术
多重离子束成像
解离放大镧系荧光免疫分析
应用举例:
Figure 1。 Goat Anti-Rabbit was labeled with Lightning-Link® 159Tb at 100 µg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.
Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link® metal labels at 10 µg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue™ (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.
Figure 3. X-Ray Fluorescence Lα series peaks used to quantify the metal content in Lightning-Link® Metal Labeling kit.
详情请联系Expedeon全国授权代理-欣博盛生物科技
全国服务热线: 4006-800-892 邮箱: market@neobioscience。com
深圳: 0755-26755892 北京: 010-88594029
上海: 021-34613729 广州:18024516375
代理品牌网站: www.nbs-bio.com
自主品牌网站: www。neobioscience。net
糖型分析网站:www。glycan-analysis。com
序号 | 最新资讯 | 浏览次数 | 作者 | 发布时间 |
1 | Adipogen热销抗体——anti-NLRP3/NALP3, mAb (Cryo-2) | 136 | 市场部 | 2019-10-30 |
2 | BioXcell新品推荐——InVivoMAb anti-mouse IL-27 p28单克隆抗体 | 306 | 市场部 | 2019-09-06 |
3 | InvivoGen 新品推荐:核酸转染增强剂——NATE™ | 362 | 市场部 | 2019-08-23 |
4 | BioXcell新品推荐——InVivoMAb anti-West Nile/dengue virus E protei | 359 | 市场部 | 2019-08-23 |
5 | 欣博盛推出新品猪ELISA试剂盒 | 629 | 市场部 | 2019-07-11 |
6 | 欣博盛ELISA试剂盒新品推荐 | 580 | 市场部 | 2019-07-11 |